Journal
/
Biochemie
/
Myosin-Specific Adaptations of In vitro Fluorescence Microscopy-Based Motility Assays
JoVE Journal
Biochemie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Biochemie
Myosin-Specific Adaptations of In vitro Fluorescence Microscopy-Based Motility Assays

Myosin-Specific Adaptations of In vitro Fluorescence Microscopy-Based Motility Assays

DOI:
10.3791/62180-v

•

08:57 min

•

February 04, 2021

•

, , , ,
1Laboratory of Molecular Physiology, Cell and Developmental Biology Center, National Heart, Lung and Blood Institute,National Institutes of Health, 2Department of Physiology, Perelman School of Medicine,University of Pennsylvania

Kapitel

  • 00:05Introduction
  • 01:04Gliding Actin Filament Assay
  • 05:02Performing the Myosin 5a TIRF Microscopy Assay
  • 06:40Results: Gliding Activity Assay of the NM2b and M5a-HMM Filaments
  • 08:05Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Presented here is a procedure to express and purify myosin 5a followed by a discussion of its characterization, using both ensemble and single molecule in vitro fluorescence microscopy-based assays, and how these methods can be modified for the characterization of nonmuscle myosin 2b.

Tags

MyosinIn VitroFluorescence MicroscopyMotility AssaysNon-muscle MyosinProtein DynamicsSingle MoleculeEnsembleCytoskeletal SystemsKinesinsDyneinsMicrotubulesNitrocelluloseFlow Chamber

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays

In Vitro Polymerization of F-actin on Early Endosomes

Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans

Quantification of Hypopigmentation Activity In Vitro

Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones

A Planarian Motility Assay to Gauge the Biomodulating Properties of Natural Products

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays

Read Article