Journal
/
Neuroscience
/
Modelado in vitro para enfermedades neurológicas utilizando la conversión directa de fibroblastos a células progenitoras neuronales y la diferenciación en astrocitos
JoVE Journal
Neuroscience
Author Produced
This content is Free Access.
JoVE Journal Neuroscience
In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes
Please note that all translations are automatically generated. Click here for the English version.

Modelado in vitro para enfermedades neurológicas utilizando la conversión directa de fibroblastos a células progenitoras neuronales y la diferenciación en astrocitos

español

Automatically Generated

4,513 Views

•

11:42 min

•

June 10, 2021

DOI:

10.3791/62016-v

DOI:
10.3791/62016-v

•

11:42 min
June 10, 2021

•

7 Views
, , , , , ,
1Center for Gene Therapy,The Research Institute at Nationwide Children’s Hospital, 2College of Medicine, The Ohio State University

Summary

Automatically generated

Se describe un protocolo para reprogramar fibroblastos derivados de la piel humana en células progenitoras neuronales inducidas (iNPCs), y su posterior diferenciación en astrocitos inducidos (iAs). Este método conduce a la generación rápida y reproducible de iNPCs e iAs en grandes cantidades.

Explore More Videos

In Vitro ModelingNeurological DiseasesFibroblastsNeuronal Progenitor CellsAstrocytesDirect ConversionSkin BiopsyRetroviral VectorsOct3/4Klf4Sox2C-MycTransductionNeurodegenerative DiseasesCell CultureCo-culturesDisease MechanismsMedication Testing

Read Article

Cite this Article

Dennys, C. N., Sierra-Delgado, J. A., Ray, S. S., Hartlaub, A. M., Roussel, F. S., Rodriguez, Y., Meyer, K. In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes. J. Vis. Exp. (172), e62016, doi:10.3791/62016 (2021).

Download .ris file

Copy

Share Video

.

Copy