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In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes
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In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes

In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes

4,512 Views

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11:42 min

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June 10, 2021

DOI:

10.3791/62016-v

DOI:
10.3791/62016-v

•

11:42 min
June 10, 2021

•

7 Views
, , , , , ,
1Center for Gene Therapy,The Research Institute at Nationwide Children’s Hospital, 2College of Medicine, The Ohio State University

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We describe a protocol to reprogram human skin-derived fibroblasts into induced Neuronal Progenitor Cells (iNPCs), and their subsequent differentiation into induced Astrocytes (iAs). This method leads to fast and reproducible generation of iNPCs and iAs in large quantities.

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Dennys, C. N., Sierra-Delgado, J. A., Ray, S. S., Hartlaub, A. M., Roussel, F. S., Rodriguez, Y., Meyer, K. In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes. J. Vis. Exp. (172), e62016, doi:10.3791/62016 (2021).

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