In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes

Cassandra N. Dennys; Julieth A. Sierra-Delgado; Shrestha Sinha Ray; Annalisa M. Hartlaub; Florence S. Roussel; Yacidzohara Rodriguez; Kathrin Meyer

doi:10.3791/62016

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In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes

JoVE Journal
神経科学
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JoVE Journal 神経科学

In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes

4,506 Views

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11:42 min

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June 10, 2021

DOI:

10.3791/62016-v

DOI:

10.3791/62016-v

•

11:42 min

June 10, 2021

•

7 Views

Cassandra N. Dennys¹, Julieth A. Sierra-Delgado¹, Shrestha Sinha Ray¹, Annalisa M. Hartlaub¹, Florence S. Roussel¹, Yacidzohara Rodriguez¹, Kathrin Meyer^1,2

¹Center for Gene Therapy,The Research Institute at Nationwide Children’s Hospital, ²College of Medicine, The Ohio State University

概要

We describe a protocol to reprogram human skin-derived fibroblasts into induced Neuronal Progenitor Cells (iNPCs), and their subsequent differentiation into induced Astrocytes (iAs). This method leads to fast and reproducible generation of iNPCs and iAs in large quantities.

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In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes

概要

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