Journal
/
Neuroscience
/
Modellazione in vitro per malattie neurologiche utilizzando la conversione diretta dai fibroblasti alle cellule progenitrici neuronali e la differenziazione in astrociti
JoVE Journal
Neuroscience
Author Produced
This content is Free Access.
JoVE Journal Neuroscience
In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes
Please note that all translations are automatically generated. Click here for the English version.

Modellazione in vitro per malattie neurologiche utilizzando la conversione diretta dai fibroblasti alle cellule progenitrici neuronali e la differenziazione in astrociti

italiano

Automatically Generated

4,513 Views

•

11:42 min

•

June 10, 2021

DOI:

10.3791/62016-v

DOI:
10.3791/62016-v

•

11:42 min
June 10, 2021

•

7 Views
, , , , , ,
1Center for Gene Therapy,The Research Institute at Nationwide Children’s Hospital, 2College of Medicine, The Ohio State University

Summary

Automatically generated

Descriviamo un protocollo per riprogrammare i fibroblasti derivati dalla pelle umana in cellule progenitrici neuronali indotte (INPC) e la loro successiva differenziazione in Astrociti indotti (iA). Questo metodo porta a una generazione rapida e riproducibile di iNPC e iA in grandi quantità.

Explore More Videos

In Vitro ModelingNeurological DiseasesFibroblastsNeuronal Progenitor CellsAstrocytesDirect ConversionSkin BiopsyRetroviral VectorsOct3/4Klf4Sox2C-MycTransductionNeurodegenerative DiseasesCell CultureCo-culturesDisease MechanismsMedication Testing

Read Article

Cite this Article

Dennys, C. N., Sierra-Delgado, J. A., Ray, S. S., Hartlaub, A. M., Roussel, F. S., Rodriguez, Y., Meyer, K. In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes. J. Vis. Exp. (172), e62016, doi:10.3791/62016 (2021).

Download .ris file

Copy

Share Video

.

Copy