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Injeção intravitreal e quantitação de parâmetros de infecção em um modelo de rato de endophtalmite bacteriana
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Immunology and Infection
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JoVE Journal Immunology and Infection
Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis

Injeção intravitreal e quantitação de parâmetros de infecção em um modelo de rato de endophtalmite bacteriana

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10,287 Views

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07:24 min

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February 06, 2021

DOI:

10.3791/61749-v

DOI:
10.3791/61749-v

•

07:24 min
February 06, 2021

•

13 Views
, , , , ,
1Department of Microbiology and Immunology,University of Oklahoma Health Sciences Center, 2Department of Ophthalmology,Dean McGee Eye Institute, 3Dean McGee Eye Institute, 4Department of Cell Biology and Department of Family and Preventive Medicine,University of Oklahoma Health Sciences Center

Summary

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Descrevemos aqui um método de injeção intravitaliana e subsequente quantitação bacteriana no modelo de camundongos de endophthalmite bacteriana. Este protocolo pode ser estendido para medir as respostas imunes do hospedeiro e a expressão genética bacteriana e hospedeira.

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Mursalin, M. H., Livingston, E., Coburn, P. S., Miller, F. C., Astley, R., Callegan, M. C. Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis. J. Vis. Exp. (168), e61749, doi:10.3791/61749 (2021).

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