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Immunology and Infection

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JoVE Journal Immunology and Infection
Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis
 

Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis

Article DOI: 10.3791/61749-v • 07:24 min • February 6th, 2021
February 6th, 2021 •
*1,2, *1, 2,3, 4, 2,3, 1,2,3
1Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, 2Department of Ophthalmology, Dean McGee Eye Institute, 3Dean McGee Eye Institute, 4Department of Cell Biology and Department of Family and Preventive Medicine, University of Oklahoma Health Sciences Center
* These authors contributed equally

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résumé
Automatic Translation

We describe here a method of intravitreal injection and subsequent bacterial quantitation in mouse model of bacterial endophthalmitis. This protocol can be extended for measuring host immune responses and bacterial and host gene expression.

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Intravitreal Injection Quantitation Infection Parameters Mouse Model Bacterial Endophthalmitis Pathogenesis Intraocular Infection Therapeutic Agents Microorganisms Host Immune Responses Gene Expression Treatment Effectiveness Visualization Needle Angle Delivery Eye Harvest
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