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Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis
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JoVE Journal 免疫学と感染
Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis

Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis

10,287 Views

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07:24 min

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February 06, 2021

DOI:

10.3791/61749-v

DOI:
10.3791/61749-v

•

07:24 min
February 06, 2021

•

13 Views
, , , , ,
1Department of Microbiology and Immunology,University of Oklahoma Health Sciences Center, 2Department of Ophthalmology,Dean McGee Eye Institute, 3Dean McGee Eye Institute, 4Department of Cell Biology and Department of Family and Preventive Medicine,University of Oklahoma Health Sciences Center

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We describe here a method of intravitreal injection and subsequent bacterial quantitation in mouse model of bacterial endophthalmitis. This protocol can be extended for measuring host immune responses and bacterial and host gene expression.

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Intravitreal InjectionMouse ModelBacterial EndophthalmitisPathogenesisIntraocular InfectionQuantitationMicroorganismsHost Immune ResponsesGene ExpressionTreatment EffectivenessBiosafety Level TwoB. CereusMicroinjectorOphthalmic MicroscopeAnesthetized MouseReverse Action Forceps

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Mursalin, M. H., Livingston, E., Coburn, P. S., Miller, F. C., Astley, R., Callegan, M. C. Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis. J. Vis. Exp. (168), e61749, doi:10.3791/61749 (2021).

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