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Iniezione intravitreale e quantificazione dei parametri di infezione in un modello di topo di endoftalmite batterica
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Immunology and Infection
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JoVE Journal Immunology and Infection
Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis
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Iniezione intravitreale e quantificazione dei parametri di infezione in un modello di topo di endoftalmite batterica

italiano

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10,287 Views

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07:24 min

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February 06, 2021

DOI:

10.3791/61749-v

DOI:
10.3791/61749-v

•

07:24 min
February 06, 2021

•

13 Views
, , , , ,
1Department of Microbiology and Immunology,University of Oklahoma Health Sciences Center, 2Department of Ophthalmology,Dean McGee Eye Institute, 3Dean McGee Eye Institute, 4Department of Cell Biology and Department of Family and Preventive Medicine,University of Oklahoma Health Sciences Center

Summary

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Descriviamo qui un metodo di iniezione intravitreale e la successiva quantificazione batterica nel modello di topo di endoftalmite batterica. Questo protocollo può essere esteso per misurare le risposte immunitarie dell'ospite e l'espressione batterica e genica ospite.

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Cite this Article

Mursalin, M. H., Livingston, E., Coburn, P. S., Miller, F. C., Astley, R., Callegan, M. C. Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis. J. Vis. Exp. (168), e61749, doi:10.3791/61749 (2021).

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