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Intravitreale Injektion und Quantifizierung von Infektionsparametern in einem Mausmodell der bakteriellen Endophthalmitis
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Immunology and Infection
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JoVE Journal Immunology and Infection
Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis
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Intravitreale Injektion und Quantifizierung von Infektionsparametern in einem Mausmodell der bakteriellen Endophthalmitis

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10,287 Views

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07:24 min

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February 06, 2021

DOI:

10.3791/61749-v

DOI:
10.3791/61749-v

•

07:24 min
February 06, 2021

•

13 Views
, , , , ,
1Department of Microbiology and Immunology,University of Oklahoma Health Sciences Center, 2Department of Ophthalmology,Dean McGee Eye Institute, 3Dean McGee Eye Institute, 4Department of Cell Biology and Department of Family and Preventive Medicine,University of Oklahoma Health Sciences Center

Summary

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Wir beschreiben hier eine Methode der intravitrealen Injektion und der anschließenden bakteriellen Quantifizierung im Mausmodell der bakteriellen Endophthalmitis. Dieses Protokoll kann erweitert werden, um die Immunantworten des Wirts und die bakterielle und die Genexpression zu messen.

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Mursalin, M. H., Livingston, E., Coburn, P. S., Miller, F. C., Astley, R., Callegan, M. C. Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis. J. Vis. Exp. (168), e61749, doi:10.3791/61749 (2021).

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