Combining Multiplex Fluorescence <em>In Situ</em> Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections

Ayse  S. Dereli; Evan  J. Bailey; Natasha  N. Kumar

doi:10.3791/61709

/

Combinando Hibridização In Situ por Fluorescência Multiplex com Imunohistoquímica Fluorescente em Seções Cerebrais de Camundongos Frescos Congelados ou Fixos

JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Neuroscience

Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections

Combinando Hibridização In Situ por Fluorescência Multiplex com Imunohistoquímica Fluorescente em Seções Cerebrais de Camundongos Frescos Congelados ou Fixos

5,698 Views

•

07:36 min

•

June 25, 2021

DOI:

10.3791/61709-v

DOI:

10.3791/61709-v

•

07:36 min

June 25, 2021

•

12 Views

Ayse S. Dereli, Evan J. Bailey, Natasha N. Kumar

¹Department of Pharmacology, School of Medical Sciences,University of New South Wales

Explore More Videos

Multiplex Fluorescence In Situ Hybridization Fluorescent Immunohistochemistry Fixed Brain Sections RNAscope Spatial Visualization MRNA Protein Neuroscience Cryostat Paraformaldehyde Fixation Dehydration Vibrating Microtome Cryoprotectant PBS Wash Hydrophobic Barrier Protease Treatment RNAscope Probes Amplification Immunohistochemistry

Read Article

Combinando Hibridização In Situ por Fluorescência Multiplex com Imunohistoquímica Fluorescente em Seções Cerebrais de Camundongos Frescos Congelados ou Fixos

Automatically Generated

Explore More Videos