/
/
Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections
5,827 Views
•
07:36 min
•
June 25, 2021
DOI:
Summary
Automatically generated
This protocol describes a method for combining fluorescence in situ hybridization (FISH) and fluorescence immunohistochemistry (IHC) in both fresh frozen and fixed mouse brain sections, with the goal of achieving multilabel FISH and fluorescence IHC signal. IHC targeted cytoplasmic and membrane attached proteins.
Explore More Videos
Multiplex Fluorescence In Situ HybridizationFluorescent ImmunohistochemistryFixed Brain SectionsRNAscopeSpatial VisualizationMRNAProteinNeurowissenschaftenCryostatParaformaldehyde FixationDehydrationVibrating MicrotomeCryoprotectantPBS WashHydrophobic BarrierProtease TreatmentRNAscope ProbesAmplificationImmunohistochemistry
Read Article
Cite this Article
Dereli, A. S., Bailey, E. J., Kumar, N. N. Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections. J. Vis. Exp. (172), e61709, doi:10.3791/61709 (2021).
Copy