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Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections
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Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections
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June 25, 2021
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Multiplex Fluorescence In Situ HybridizationFluorescent ImmunohistochemistryFixed Brain SectionsRNAscopeSpatial VisualizationMRNAProteinNeuroscienzeCryostatParaformaldehyde FixationDehydrationVibrating MicrotomeCryoprotectantPBS WashHydrophobic BarrierProtease TreatmentRNAscope ProbesAmplificationImmunohistochemistry
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Dereli, A. S., Bailey, E. J., Kumar, N. N. Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections. J. Vis. Exp. (172), e61709, doi:10.3791/61709 (2021).
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