Combining Multiplex Fluorescence <em>In Situ</em> Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections

Ayse  S. Dereli; Evan  J. Bailey; Natasha  N. Kumar

doi:10.3791/61709

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Combinaison de l’hybridation in situ par fluorescence multiplex avec l’immunohistochimie fluorescente sur des coupes de cerveau de souris fraîches, congelées ou fixes

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Neuroscience

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JoVE Journal Neuroscience

Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections

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Combinaison de l’hybridation in situ par fluorescence multiplex avec l’immunohistochimie fluorescente sur des coupes de cerveau de souris fraîches, congelées ou fixes

5,698 Views

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07:36 min

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June 25, 2021

DOI:

10.3791/61709-v

DOI:

10.3791/61709-v

•

07:36 min

June 25, 2021

•

12 Views

Ayse S. Dereli, Evan J. Bailey, Natasha N. Kumar

¹Department of Pharmacology, School of Medical Sciences,University of New South Wales

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Multiplex Fluorescence In Situ Hybridization Fluorescent Immunohistochemistry Fixed Brain Sections RNAscope Spatial Visualization MRNA Protein Neuroscience Cryostat Paraformaldehyde Fixation Dehydration Vibrating Microtome Cryoprotectant PBS Wash Hydrophobic Barrier Protease Treatment RNAscope Probes Amplification Immunohistochemistry

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Combinaison de l’hybridation in situ par fluorescence multiplex avec l’immunohistochimie fluorescente sur des coupes de cerveau de souris fraîches, congelées ou fixes

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