Combining Multiplex Fluorescence <em>In Situ</em> Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections

Ayse  S. Dereli; Evan  J. Bailey; Natasha  N. Kumar

doi:10.3791/61709

/

Combinazione dell'ibridazione in situ a fluorescenza multiplex con immunoistochimica fluorescente su sezioni cerebrali di topo fresche, congelate o fisse

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Neuroscience

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JoVE Journal Neuroscience

Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections

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Combinazione dell'ibridazione in situ a fluorescenza multiplex con immunoistochimica fluorescente su sezioni cerebrali di topo fresche, congelate o fisse

5,698 Views

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07:36 min

•

June 25, 2021

DOI:

10.3791/61709-v

DOI:

10.3791/61709-v

•

07:36 min

June 25, 2021

•

12 Views

Ayse S. Dereli, Evan J. Bailey, Natasha N. Kumar

¹Department of Pharmacology, School of Medical Sciences,University of New South Wales

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Multiplex Fluorescence In Situ Hybridization Fluorescent Immunohistochemistry Fixed Brain Sections RNAscope Spatial Visualization MRNA Protein Neuroscience Cryostat Paraformaldehyde Fixation Dehydration Vibrating Microtome Cryoprotectant PBS Wash Hydrophobic Barrier Protease Treatment RNAscope Probes Amplification Immunohistochemistry

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Combinazione dell'ibridazione in situ a fluorescenza multiplex con immunoistochimica fluorescente su sezioni cerebrali di topo fresche, congelate o fisse

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