Journal
/
Genetics
/
In Vivo CRISPR/Cas9 Screening para evaluar simultáneamente la función génica en la piel del ratón y la cavidad oral
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
Please note that all translations are automatically generated. Click here for the English version.

In Vivo CRISPR/Cas9 Screening para evaluar simultáneamente la función génica en la piel del ratón y la cavidad oral

español

Automatically Generated

6,119 Views

•

07:52 min

•

November 02, 2020

DOI:

10.3791/61693-v

DOI:
10.3791/61693-v

•

07:52 min
November 02, 2020

•

13 Views
, , , ,
1Centre for Molecular and Systems Biology,Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 2Department of Molecular Genetics,University of Toronto, 3Department of Cell and Developmental Biology, Sackler Faculty of Medicine,Tel Aviv University

Summary

Automatically generated

Aquí describimos una metodología de detección CRISPR/Cas9 rápida y directa in vivo utilizando inyecciones lentivirales embrionarias de tero guiadas por ultrasonido para evaluar simultáneamente las funciones de varios genes en la piel y la cavidad oral de ratones inmunocompetentntes.

Explore More Videos

CRISPR/Cas9In Vivo ScreeningMouse SkinOral CavityTumor Suppressor GenesHead And Neck CancerGene FunctionSkin ExpressionTargeted CRISPR LibraryPregnancyEmbryonic Day 9.5UltrasoundAnesthesiaIncisionPeritoneumUterine HornSilicone MembranePBSModeling Clay

Read Article

Cite this Article

Loganathan, S. K., Malik, A., Langille, E., Luxenburg, C., Schramek, D. In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity. J. Vis. Exp. (165), e61693, doi:10.3791/61693 (2020).

Download .ris file

Copy

Share Video

.

Copy