In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Sampath  Kumar Loganathan; Ahmad Malik; Ellen Langille; Chen Luxenburg; Daniel Schramek

doi:10.3791/61693

Journal

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Genética

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In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

JoVE Journal
Genética

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JoVE Journal Genética

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

6,121 Views

•

07:52 min

•

November 02, 2020

DOI:

10.3791/61693-v

DOI:

10.3791/61693-v

•

07:52 min

November 02, 2020

•

13 Views

Sampath Kumar Loganathan¹, Ahmad Malik^1,2, Ellen Langille^1,2, Chen Luxenburg³, Daniel Schramek^1,2

¹Centre for Molecular and Systems Biology,Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, ²Department of Molecular Genetics,University of Toronto, ³Department of Cell and Developmental Biology, Sackler Faculty of Medicine,Tel Aviv University

Summary

Here we describe a rapid and direct in vivo CRISPR/Cas9 screening methodology using ultrasound-guided in utero embryonic lentiviral injections to simultaneously assess functions of several genes in the skin and oral cavity of immunocompetent mice.

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In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

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