/

/

In Vivo CRISPR/Cas9スクリーニングによるマウス皮膚と口腔の遺伝子機能の同時評価

JoVE Journal
Genetics

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Genetics

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Please note that all translations are automatically generated. Click here for the English version.

In Vivo CRISPR/Cas9スクリーニングによるマウス皮膚と口腔の遺伝子機能の同時評価

6,119 Views

•

07:52 min

•

November 02, 2020

DOI:

10.3791/61693-v

DOI:

10.3791/61693-v

•

07:52 min

November 02, 2020

•

13 Views

Sampath Kumar Loganathan¹, Ahmad Malik^1,2, Ellen Langille^1,2, Chen Luxenburg³, Daniel Schramek^1,2

¹Centre for Molecular and Systems Biology,Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, ²Department of Molecular Genetics,University of Toronto, ³Department of Cell and Developmental Biology, Sackler Faculty of Medicine,Tel Aviv University

Summary

ここでは、子宮胚性レンチウイルス注射で超音波誘導を使用した迅速かつ直接的なin vivo CRISPR/Cas9スクリーニング方法論を説明し、免疫担当マウスの皮膚および口腔内のいくつかの遺伝子の機能を同時に評価する。

Explore More Videos

CRISPR/Cas9 In Vivo Screening Mouse Skin Oral Cavity Tumor Suppressor Genes Head And Neck Cancer Gene Function Skin Expression Targeted CRISPR Library Pregnancy Embryonic Day 9.5 Ultrasound Anesthesia Incision Peritoneum Uterine Horn Silicone Membrane PBS Modeling Clay

Read Article