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In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
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Genetik
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JoVE Journal Genetik
In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

6,121 Views

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07:52 min

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November 02, 2020

DOI:

10.3791/61693-v

DOI:
10.3791/61693-v

•

07:52 min
November 02, 2020

•

13 Views
, , , ,
1Centre for Molecular and Systems Biology,Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 2Department of Molecular Genetics,University of Toronto, 3Department of Cell and Developmental Biology, Sackler Faculty of Medicine,Tel Aviv Üniversitesi

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Here we describe a rapid and direct in vivo CRISPR/Cas9 screening methodology using ultrasound-guided in utero embryonic lentiviral injections to simultaneously assess functions of several genes in the skin and oral cavity of immunocompetent mice.

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Loganathan, S. K., Malik, A., Langille, E., Luxenburg, C., Schramek, D. In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity. J. Vis. Exp. (165), e61693, doi:10.3791/61693 (2020).

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