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In Vivo CRISPR/Cas9-screening om tegelijkertijd de genfunctie in de huid en mondholte van de muis te evalueren
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Genetics
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JoVE Journal Genetics
In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
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In Vivo CRISPR/Cas9-screening om tegelijkertijd de genfunctie in de huid en mondholte van de muis te evalueren

Nederlands

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6,119 Views

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07:52 min

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November 02, 2020

DOI:

10.3791/61693-v

DOI:
10.3791/61693-v

•

07:52 min
November 02, 2020

•

13 Views
, , , ,
1Centre for Molecular and Systems Biology,Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 2Department of Molecular Genetics,University of Toronto, 3Department of Cell and Developmental Biology, Sackler Faculty of Medicine,Tel Aviv University

Summary

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Hier beschrijven we een snelle en directe in vivo CRISPR/Cas9 screeningsmethodologie met behulp van echogeleide in utero embryonale lentivirale injecties om tegelijkertijd functies van verschillende genen in de huid en mondholte van immunocompetente muizen te beoordelen.

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Cite this Article

Loganathan, S. K., Malik, A., Langille, E., Luxenburg, C., Schramek, D. In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity. J. Vis. Exp. (165), e61693, doi:10.3791/61693 (2020).

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