Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes

Victoria Osinski; Alexander  L Klibanov; Coleen  A McNamara

doi:10.3791/61585

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Préparation, administration et évaluation de l’absorption cellulaire in vivo de liposomes marqués par un colorant fluorescent

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes

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Préparation, administration et évaluation de l’absorption cellulaire in vivo de liposomes marqués par un colorant fluorescent

3,324 Views

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08:44 min

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July 30, 2020

DOI:

10.3791/61585-v

DOI:

10.3791/61585-v

•

08:44 min

July 30, 2020

•

44 Views

Victoria Osinski^1,2, Alexander L Klibanov^1,3, Coleen A McNamara^1,3

¹Robert M. Berne Cardiovascular Research Center,University of Virginia, ²Department of Pathology,University of Virginia, ³Department of Medicine, Division of Cardiovascular Medicine,University of Virginia

Summary

L’objectif de ce protocole est de synthétiser des liposomes marqués par fluorescence et d’utiliser la cytométrie en flux pour identifier la localisation in vivo des liposomes au niveau cellulaire.

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Préparation, administration et évaluation de l’absorption cellulaire in vivo de liposomes marqués par un colorant fluorescent

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Summary

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