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Bioengineering
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荧光染料标记脂质体体内组织特异性细胞摄取的制备、给药和评估
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes
Please note that all translations are automatically generated.
Click here for the English version.
荧光染料标记脂质体体内组织特异性细胞摄取的制备、给药和评估
DOI:
10.3791/61585-v
•
08:44 min
•
July 30, 2020
•
Victoria Osinski
2
,
Alexander L Klibanov
3
,
Coleen A McNamara
3
1
Robert M. Berne Cardiovascular Research Center
,
University of Virginia
,
2
Department of Pathology
,
University of Virginia
,
3
Department of Medicine, Division of Cardiovascular Medicine
,
University of Virginia
Chapters
00:05
Introduction
00:37
Fluorescent Liposome Labeling and Loading
01:59
Liposome Administration
02:25
Post‐Mortem Tissue Harvest
04:54
Adipose Tissue Processing
06:33
Results: Liposome Identification, Uptake, and Analysis
08:06
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议的目标是合成荧光标记的脂质体,并使用流式细胞术在细胞水平上鉴定脂质体的体内定位。
Tags
Liposome Preparation
Fluorescent Dye Labeling
Tesaglitazar
In Vivo Cellular Uptake
Retro-orbital Injection
Blood Sampling
Dynamic Light Scattering
Spectrophotometry
Emulsification
Rotary Evaporation
Membrane Extrusion
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