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Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes
JoVE Journal
Biotechnik
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JoVE Journal Biotechnik
Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes

Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes

DOI:
10.3791/61585-v

•

08:44 min

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July 30, 2020

•

, ,
1Robert M. Berne Cardiovascular Research Center,University of Virginia, 2Department of Pathology,University of Virginia, 3Department of Medicine, Division of Cardiovascular Medicine,University of Virginia

Kapitel

  • 00:05Introduction
  • 00:37Fluorescent Liposome Labeling and Loading
  • 01:59Liposome Administration
  • 02:25Post‐Mortem Tissue Harvest
  • 04:54Adipose Tissue Processing
  • 06:33Results: Liposome Identification, Uptake, and Analysis
  • 08:06Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

The goal of this protocol is to synthesize fluorescently-labeled liposomes and use flow cytometry to identify in vivo localization of liposomes at a cellular level.

Tags

Liposome PreparationFluorescent Dye LabelingTesaglitazarIn Vivo Cellular UptakeRetro-orbital InjectionBlood SamplingDynamic Light ScatteringSpectrophotometryEmulsificationRotary EvaporationMembrane Extrusion

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