Journal
/
Bioengenharia
/
Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes
JoVE Journal
Bioengenharia
This content is Free Access.
JoVE Journal Bioengenharia
Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes

Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes

3,317 Views

•

08:44 min

•

July 30, 2020

DOI:

10.3791/61585-v

DOI:
10.3791/61585-v

•

08:44 min
July 30, 2020

•

44 Views
, ,
1Robert M. Berne Cardiovascular Research Center,University of Virginia, 2Department of Pathology,University of Virginia, 3Department of Medicine, Division of Cardiovascular Medicine,University of Virginia

Summary

Automatically generated

The goal of this protocol is to synthesize fluorescently-labeled liposomes and use flow cytometry to identify in vivo localization of liposomes at a cellular level.

Explore More Videos

Liposome PreparationFluorescent Dye LabelingTesaglitazarIn Vivo Cellular UptakeRetro-orbital InjectionBlood SamplingDynamic Light ScatteringSpectrophotometryEmulsificationRotary EvaporationMembrane Extrusion

Read Article

Cite this Article

Osinski, V., Klibanov, A. L., McNamara, C. A. Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes. J. Vis. Exp. (161), e61585, doi:10.3791/61585 (2020).

Download .ris file

Copy

Share Video

.

Copy