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Biochemistry
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实时测量核苷酸与完整功能性膜蛋白的结合
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time
Please note that all translations are automatically generated.
Click here for the English version.
实时测量核苷酸与完整功能性膜蛋白的结合
DOI:
10.3791/61401-v
•
08:33 min
•
March 11, 2021
•
Samuel G. Usher
,
Frances M. Ashcroft
,
Michael C. Puljung
2
1
Department of Physiology, Anatomy and Genetics
,
University of Oxford
,
2
Department of Chemistry and Neuroscience Program
,
Trinity College
Chapters
00:04
Introduction
00:36
Unroofed Membrane Experiments
04:36
Patch-Clamp Fluorometry Experiment
05:56
Results: Representative Measurement of Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time
07:55
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议提出了一种在细胞环境中实时测量腺嘌呤核苷酸与受体结合的方法。结合测量为三硝基苯基核苷酸衍生物与用非规范荧光氨基酸标记的蛋白质之间的Förster共振能量转移(FRET)。
Tags
Nucleotide Binding
Membrane Proteins
FRET
ATP
Potassium Channel
Diabetes
Unroofed Membrane
Sonication
Microscopy
ANAP
Fluorescence Imaging
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