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Measuring Protein Binding to F-actin by Co-sedimentation
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Measuring Protein Binding to F-actin by Co-sedimentation

Measuring Protein Binding to F-actin by Co-sedimentation

DOI:
10.3791/55613-v

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06:17 min

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May 18, 2017

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, ,
1Department of Cell Biology,University of Pittsburgh School of Medicine, 2Department of Biology,University of North Carolina at Chapel Hill

Chapters

  • 00:05Title
  • 00:33Prepare the Test Protein and F-actin for the Assay
  • 02:02Pelleting Assay – Quantification
  • 04:33Results: αE-catenin Homodimer Binds F-actin
  • 05:16Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a method to test the ability of a protein to co-sediment with filamentous actin (F-actin) and, if binding is observed, to measure the affinity of the interaction.

Tags

Protein BindingF-actinCo-sedimentationActin PolymerizationUltracentrifugationProtein ConcentrationBinding AssayAffinity Measurement

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