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Measuring Protein Binding to F-actin by Co-sedimentation
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Biochemistry
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JoVE Journal Biochemistry
Measuring Protein Binding to F-actin by Co-sedimentation

Measuring Protein Binding to F-actin by Co-sedimentation

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16,020 Views

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06:17 min

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May 18, 2017

DOI:

10.3791/55613-v

DOI:
10.3791/55613-v

•

06:17 min
May 18, 2017

•

18 Views
, ,
1Department of Cell Biology,University of Pittsburgh School of Medicine, 2Department of Biology,University of North Carolina at Chapel Hill

Summary

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This protocol describes a method to test the ability of a protein to co-sediment with filamentous actin (F-actin) and, if binding is observed, to measure the affinity of the interaction.

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Cite this Article

Heier, J. A., Dickinson, D. J., Kwiatkowski, A. V. Measuring Protein Binding to F-actin by Co-sedimentation. J. Vis. Exp. (123), e55613, doi:10.3791/55613 (2017).

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