Journal
/
生物化学
/
Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time
JoVE 杂志
生物化学
This content is Free Access.
JoVE 杂志 生物化学
Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time

1,614 Views

•

08:33 min

•

March 11, 2021

DOI:

10.3791/61401-v

DOI:
10.3791/61401-v

•

08:33 min
March 11, 2021

•

3 Views
, ,
1Department of Physiology, Anatomy and Genetics,University of Oxford, 2Department of Chemistry and Neuroscience Program,Trinity College

Tags

Nucleotide BindingMembrane ProteinsFRETATPPotassium ChannelDiabetesUnroofed MembraneSonicationMicroscopyANAPFluorescence Imaging

Read Article

Cite this Article

Usher, S. G., Ashcroft, F. M., Puljung, M. C. Measuring Nucleotide Binding to Intact, Functional Membrane Proteins in Real Time. J. Vis. Exp. (169), e61401, doi:10.3791/61401 (2021).

Download .ris file

Copy

Share Video

.

Copy