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Fluorescerende immunolocalisatie van Arabinogalactan-eiwitten en pectines in de celwand van plantenweefsels
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Developmental Biology
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JoVE Journal Developmental Biology
Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues
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Fluorescerende immunolocalisatie van Arabinogalactan-eiwitten en pectines in de celwand van plantenweefsels

Nederlands

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5,830 Views

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10:14 min

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February 27, 2021

DOI:

10.3791/61034-v

DOI:
10.3791/61034-v

•

10:14 min
February 27, 2021

•

20 Views
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1Departamento de Biologia,Faculdade de Ciências da Universidade do Porto, 2Laboratório Associado para a Química Verde, 3Dipartimento di Bioscienze,Universitá degli Studi di Milano

Summary

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Dit protocol beschrijft in detail hoe het plantaardige materiaal voor immunolocalisatie van Arabinogalactan-eiwitten en pectines is bevestigd, ingebed in een hydrofiele acrylhars, doorsneden en gemonteerd op glazen dia's. We laten zien dat celwandgerelateerde epitopen worden gedetecteerd met specifieke antilichamen.

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Costa, M., Pereira, A. M., Coimbra, S. Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues. J. Vis. Exp. (168), e61034, doi:10.3791/61034 (2021).

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