Journal
/
Biochemistry
/
Conventionele BODIPY Conjugates voor Live-Cell Super-Resolution Microscopie en Single-Molecule Tracking
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
Please note that all translations are automatically generated. Click here for the English version.

Conventionele BODIPY Conjugates voor Live-Cell Super-Resolution Microscopie en Single-Molecule Tracking

Nederlands

Automatically Generated

7,957 Views

•

07:49 min

•

June 08, 2020

DOI:

10.3791/60950-v

DOI:
10.3791/60950-v

•

07:49 min
June 08, 2020

•

5 Views
, , ,
1School of Physics and Astronomy,University of Minnesota, Twin Cities, Physics and Nanotechnology (PAN), 2Middlebury College

Summary

Automatically generated

Conventionele BODIPY conjugaten kunnen worden gebruikt voor live-cel single-molecule lokalisatie microscopie (SMLM) door de exploitatie van hun tijdelijk vormende, rood verschoven grond staat dimers. We presenteren een geoptimaliseerd SMLM-protocol om subcellulaire neutrale lipiden en vetzuren in levende zoogdier- en gistcellen op nanoscopische lengteschaal te volgen en op te lossen.

Explore More Videos

BODIPY ConjugatesSuper-resolution MicroscopySingle-molecule TrackingLive-cell ImagingLipid DropletsFatty AcidYeast CellsMammalian CellsEMCCD CameraLaser PowerMicroscope Optimization

Read Article

Cite this Article

Adhikari, S., Banerjee, C., Moscatelli, J., Puchner, E. M. Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking. J. Vis. Exp. (160), e60950, doi:10.3791/60950 (2020).

Download .ris file

Copy

Share Video

.

Copy