Journal
/
Biochemistry
/
OaAEP1-媒介酵素合成と単分子力分光法のための重合タンパク質の固定化
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
Please note that all translations are automatically generated. Click here for the English version.

OaAEP1-媒介酵素合成と単分子力分光法のための重合タンパク質の固定化

日本語

Automatically Generated

6,547 Views

•

08:34 min

•

February 05, 2020

DOI:

10.3791/60774-v

DOI:
10.3791/60774-v

•

08:34 min
February 05, 2020

•

4 Views
, , , , , ,
1State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering,Nanjing University

Summary

Automatically generated

ここでは、タンパク質ポリマーを制御された配列で形成する酵素によってタンパク質モノマーを結合し、単一分子力分光研究のために表面に固定化するプロトコルを提示する。

Explore More Videos

OaAEP1Enzymatic SynthesisPolymerized ProteinSingle-molecule Force SpectroscopyProtein OligomerPolymerization DegreeProtein-based MaterialCysteinePotassium ChromateSulfuric AcidAPTESSulfo-SMCCGL-ELP-50 Cys-proteinCantilever Surface

Read Article

Cite this Article

Deng, Y., Zheng, B., Liu, Y., Shi, S., Nie, J., Wu, T., Zheng, P. OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy. J. Vis. Exp. (156), e60774, doi:10.3791/60774 (2020).

Download .ris file

Copy

Share Video

.

Copy