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Immunology and Infection
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Prædiktiv immunmodellering af solide tumorer
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Predictive Immune Modeling of Solid Tumors

Prædiktiv immunmodellering af solide tumorer

DOI:
10.3791/60645-v

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08:50 min

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February 25, 2020

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, ,
1Cofactor Genomics

Chapters

  • 00:05Introduction
  • 01:00First Strand DNA Synthesis
  • 01:44Blocking Oligos, Cot-1 DNA, and Pre-Capture Library Preparation for Hybridization
  • 02:12Hybridized Target Streptavidin Bead Binding
  • 02:54Streptavidin Bead Unbound DNA Removal
  • 06:39Results: Representative Predictive Immune Modeling
  • 08:03Conclusion

Summary

Automatic Translation

Automatic Translation

Brugen af en RNA-baseret tilgang til at bestemme kvantitative immunprofiler af fast tumorvæv og udnytte kliniske kohorter til immun-onkologi biomarkør opdagelse er beskrevet gennem en molekylær og informatik protokoller.

Tags

Predictive Immune ModelingSolid TumorsTumor MicroenvironmentImmune CellsRNA-based Expression ModelsImmuno-oncologyFormalin-fixed Paraffin EmbeddedRNA Library PreparationTargeted CaptureFirst Strand SynthesisCOT-1 DNABlocking OligosHybridizationMagnetic BeadsWash Buffer

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