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Immunology and Infection
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Predictive Immune Modeling of Solid Tumors
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Predictive Immune Modeling of Solid Tumors

Predictive Immune Modeling of Solid Tumors

English

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6,621 Views

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08:50 min

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February 25, 2020

DOI:

10.3791/60645-v

DOI:
10.3791/60645-v

•

08:50 min
February 25, 2020

•

2 Views
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1Cofactor Genomics

Summary

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The use of an RNA-based approach to determine quantitative immune profiles of solid tumor tissues and leverage clinical cohorts for immune-oncology biomarker discovery is described through a molecular and informatics protocols.

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Predictive Immune ModelingSolid TumorsTumor MicroenvironmentImmune CellsRNA-based Expression ModelsImmuno-oncologyFormalin-fixed Paraffin EmbeddedRNA Library PreparationTargeted CaptureFirst Strand SynthesisCOT-1 DNABlocking OligosHybridizationMagnetic BeadsWash Buffer

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LaFranzo, N. A., Flanagan, K. C., Quintanilha, D. Predictive Immune Modeling of Solid Tumors. J. Vis. Exp. (156), e60645, doi:10.3791/60645 (2020).

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