Journal
/
면역학 및 감염병학
/
Predictive Immune Modeling of Solid Tumors
JoVE 신문
면역학 및 감염병학
This content is Free Access.
JoVE 신문 면역학 및 감염병학
Predictive Immune Modeling of Solid Tumors

Predictive Immune Modeling of Solid Tumors

DOI:
10.3791/60645-v

•

08:50 min

•

February 25, 2020

•

, ,
1Cofactor Genomics

Chapters

  • 00:05Introduction
  • 01:00First Strand DNA Synthesis
  • 01:44Blocking Oligos, Cot-1 DNA, and Pre-Capture Library Preparation for Hybridization
  • 02:12Hybridized Target Streptavidin Bead Binding
  • 02:54Streptavidin Bead Unbound DNA Removal
  • 06:39Results: Representative Predictive Immune Modeling
  • 08:03Conclusion

Summary

자동 번역

자동 번역

The use of an RNA-based approach to determine quantitative immune profiles of solid tumor tissues and leverage clinical cohorts for immune-oncology biomarker discovery is described through a molecular and informatics protocols.

Tags

Predictive Immune ModelingSolid TumorsTumor MicroenvironmentImmune CellsRNA-based Expression ModelsImmuno-oncologyFormalin-fixed Paraffin EmbeddedRNA Library PreparationTargeted CaptureFirst Strand SynthesisCOT-1 DNABlocking OligosHybridizationMagnetic BeadsWash Buffer

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry

Seven Steps to Stellate Cells

Flow Cytometry Analysis of Immune Cells Within Murine Aortas

Isolation of Functional Cardiac Immune Cells

Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy

Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation

Isolation of Adipose Tissue Immune Cells

Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

Isolation and Identification of Extravascular Immune Cells of the Heart

Read Article