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Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation
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Immunology and Infection
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JoVE Journal Immunology and Infection
Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation

Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation

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17,264 Views

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14:43 min

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October 01, 2012

DOI:

10.3791/4208-v

DOI:
10.3791/4208-v

•

14:43 min
October 01, 2012

•

8 Views
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1Center for Inflammatory Bowel Diseases, Division of Digestive Diseases, David Geffen School of Medicine,The University of California Los Angeles, Los Angeles

Summary

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Bone marrow transplantation provides a way to change the genotype of the bone marrow derived cells. If the gene of interest is expressed in both bone marrow derived cells and non-bone marrow derived cells, bone marrow transplantation can change the bone marrow derived cells to a different genotype without changing the non-bone marrow derived cell genotype.

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Gene ExpressionImmune CellsNon-immune CellsMouse ColitisBone Marrow TransplantationGene-of-interestKnockout MiceGenotypeIrradiationDonor Bone MarrowRecipient MiceWild-type MiceCD Molecules (CD45.1 And CD45.2)

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Koon, H. W., Ho, S., Cheng, M., Ichikawa, R., Pothoulakis, C. Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation. J. Vis. Exp. (68), e4208, doi:10.3791/4208 (2012).

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