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Immunology and Infection

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固体肿瘤的预测免疫建模

JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection

Predictive Immune Modeling of Solid Tumors

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固体肿瘤的预测免疫建模

DOI:

10.3791/60645-v

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08:50 min

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February 25, 2020

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Natalie A. LaFranzo, Kevin C. Flanagan, Danielle Quintanilha

¹Cofactor Genomics

Chapters

00:05Introduction
01:00First Strand DNA Synthesis
01:44Blocking Oligos, Cot-1 DNA, and Pre-Capture Library Preparation for Hybridization
02:12Hybridized Target Streptavidin Bead Binding
02:54Streptavidin Bead Unbound DNA Removal
06:39Results: Representative Predictive Immune Modeling
08:03Conclusion

Summary

Automatic Translation

通过分子和信息学协议描述使用基于RNA的方法确定固体肿瘤组织的定量免疫特征，并利用临床队列进行免疫肿瘤学生物标志物发现。

Tags

Predictive Immune Modeling Solid Tumors Tumor Microenvironment Immune Cells RNA-based Expression Models Immuno-oncology Formalin-fixed Paraffin Embedded RNA Library Preparation Targeted Capture First Strand Synthesis COT-1 DNA Blocking Oligos Hybridization Magnetic Beads Wash Buffer

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