Journal
/
Developmental Biology
/
斑马鱼胚胎全山原位杂交和管状测定在iPSC-ECs中研究恩多林在血管发育中的作用
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Whole-Mount In Situ Hybridization in Zebrafish Embryos and Tube Formation Assay in iPSC-ECs to Study the Role of Endoglin in Vascular Development
Please note that all translations are automatically generated. Click here for the English version.

斑马鱼胚胎全山原位杂交和管状测定在iPSC-ECs中研究恩多林在血管发育中的作用

DOI:
10.3791/60498-v

•

08:27 min

•

May 28, 2020

•

, , , , , ,
1Department of Physiology and Department of Cardiology of the Second Affiliated Hospital,Zhejiang University School of Medicine, 2Department of Cell Biology, State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences and Peking Union Medical College, 3Wuxi Lung Transplant Center,Wuxi People’s Hospital affiliated to Nanjing Medical University

Chapters

  • 00:04Introduction
  • 01:00Zebrafish Embryo Collection and Fixation
  • 02:55Amplification and Microscopy
  • 04:27Tube Formation Assay
  • 06:40Results: Defective Tube Formation After Endoglin Knockdown
  • 07:43Conclusion

Summary

Automatic Translation

Automatic Translation

本文介绍的是一种在斑马鱼和管状细胞中对斑马鱼进行全位RNA杂交分析的协议，在患者诱导的多能干细胞衍生内皮细胞中进行管状分析，以研究内索林在血管形成中的作用。

Tags

Whole-mount In Situ HybridizationWISHZebrafish EmbryosTube Formation AssayIPSC-ECsEndoglinVascular DevelopmentMicroinjectionMorpholinosMRNAProteinase KTarget ProbesParaformaldehydeSSCTAmp 1Amp 2

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

斑马鱼幼虫骨骼肌诚信与伊文思蓝分析

多目标显色全安装<em>原位</em>在比较基因表达域杂交<em>果蝇</em>胚胎

成人果蝇脑内蘑菇体和感光神经元的解剖和荧光染色

全贴装荧光原位杂交和免疫荧光法在斑马鱼中 Multiciliated 细胞的可视化

小鼠胚胎心肌内祖群的定量全贴免疫荧光分析

RANKL 小鼠骨髓破骨细胞培养法研究 mTORC1 在破骨细胞形成中的作用

用扫描电镜和全贴免疫荧光技术可视化小鼠胚胎中的节点和外型板

阿斯蒂亚纳克斯胚胎的全原位杂交

斑马鱼原位杂交染色的基因分型与定量

斑马鱼胚胎和幼虫的整个山免疫性化学

Read Article