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Whole-Mount In Situ Hybridization in Zebrafish Embryos and Tube Formation Assay in iPSC-ECs to Study the Role of Endoglin in Vascular Development
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Whole-Mount In Situ Hybridization in Zebrafish Embryos and Tube Formation Assay in iPSC-ECs to Study the Role of Endoglin in Vascular Development
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08:27 min
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May 28, 2020
DOI:
1Department of Physiology and Department of Cardiology of the Second Affiliated Hospital,Zhejiang University School of Medicine, 2Department of Cell Biology, State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences and Peking Union Medical College, 3Wuxi Lung Transplant Center,Wuxi People’s Hospital affiliated to Nanjing Medical University
Summary
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Presented here is a protocol for whole-mount in situ RNA hybridization analysis in zebrafish and tube formation assay in patient-derived induced pluripotent stem cell-derived endothelial cells to study the role of endoglin in vascular formation.
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Wang, Y., Zhang, D., Zhou, F., Zhou, M., Li, Q., Chen, J., Yang, J. Whole-Mount In Situ Hybridization in Zebrafish Embryos and Tube Formation Assay in iPSC-ECs to Study the Role of Endoglin in Vascular Development. J. Vis. Exp. (159), e60498, doi:10.3791/60498 (2020).
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