Journal
/
Developmental Biology
/
Wholemount In Situ Hybridization for Astyanax Embryos
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Wholemount In Situ Hybridization for Astyanax Embryos

Wholemount In Situ Hybridization for Astyanax Embryos

DOI:
10.3791/59114-v

•

09:56 min

•

March 02, 2019

•

, , ,
1Department of Biological Sciences,University of Cincinnati

Chapters

  • 00:04Title
  • 00:40Day 1: Rehydration
  • 01:30Day 1: Digestion and Fixation
  • 02:42Day 1: Prehybridization and Day 2: Hybridization
  • 03:46Day 3: Solution Preparation and Probe Removal
  • 05:02Day 3: Blocking
  • 05:42Day 4: MABT Rinses
  • 06:34Day 5: Probe Visualization
  • 08:00Results: Analysis of Mounted Astyanax Embryos
  • 09:37Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol enables visualization of gene expression in embryonic Astyanax cavefish. This approach has been developed with the goal of maximizing gene expression signal, while minimizing non-specific background staining.

Tags

Keywords: Wholemount In Situ HybridizationAstyanax EmbryosGene Expression PatternsProtocolPlatform ShakerProteinase KPBTPFAHybridization BufferWater Bath

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59114?article_type=v

Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus
check_url/59114?article_type=v

Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos
check_url/59114?article_type=v

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence
check_url/59114?article_type=v

Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes
check_url/59114?article_type=v

Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors
check_url/59114?article_type=v

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence
check_url/59114?article_type=v

Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish
check_url/59114?article_type=v

Whole-Mount In Situ Hybridization in Zebrafish Embryos and Tube Formation Assay in iPSC-ECs to Study the Role of Endoglin in Vascular Development
check_url/59114?article_type=v

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis
check_url/59114?article_type=v

Incremental Temperature Changes for Maximal Breeding and Spawning in Astyanax mexicanus

Read Article