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通过清除心脏三维成像捕获目标细胞群的心脏损伤反应
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Developmental Biology
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JoVE Journal Developmental Biology
Capturing the Cardiac Injury Response of Targeted Cell Populations via Cleared Heart Three-Dimensional Imaging
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通过清除心脏三维成像捕获目标细胞群的心脏损伤反应

DOI:
10.3791/60482-v

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08:14 min

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March 17, 2020

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, ,
1Department of Cell and Regenerative Biology,University of Wisconsin-Madison School of Medicine and Public Health

Chapters

  • 00:05Introduction
  • 00:52Left Anterior Descending Artery (LAD) Ligation-Induced Coronary Artery Occlusion (Myocardial Infarction)
  • 02:58Passive Clear Lipid-Exchanged Acrylamide-Hybridized Rigid Imaging Compatible Tissue Hydrogel (CLARITY)
  • 05:53Single-Photon Confocal Microscopy Cleared Mouse Heart Imaging
  • 06:28Results: Representative Neonatal Mouse Visualization
  • 07:46Conclusion

Summary

Automatic Translation

Automatic Translation

伤愈后心肌细胞增殖是一个动态过程，需要来自非肌细胞群的细胞外线索交响。利用谱系跟踪、被动CLARITY和三维全贴装共聚焦显微镜技术，可以分析各种细胞类型对心脏修复和再生的影响。

Tags

Cardiac InjuryCell PopulationsThree-dimensional ImagingHeart RegenerationLAD OcclusionMyocardial InfarctionFluorescent StainingMicroscopy

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