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Journal / Developmental Biology / Capturing the Cardiac Injury Response of Targeted …
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Developmental Biology

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JoVE Journal Developmental Biology
Capturing the Cardiac Injury Response of Targeted Cell Populations via Cleared Heart Three-Dimensional Imaging
 

Capturing the Cardiac Injury Response of Targeted Cell Populations via Cleared Heart Three-Dimensional Imaging

Article DOI: 10.3791/60482-v • 08:14 min • March 17th, 2020
March 17th, 2020 •
1, 1, 1
1Department of Cell and Regenerative Biology, University of Wisconsin-Madison School of Medicine and Public Health

Kapitel

Summary
Automatic Translation

Cardiomyocyte proliferation following injury is a dynamic process that requires a symphony of extracellular cues from non-myocyte cell populations. Utilizing lineage tracing, passive CLARITY, and three-dimensional whole-mount confocal microscopy techniques, we can analyze the influence of a variety of cell types on cardiac repair and regeneration.

Tags

Cardiac Injury Response Targeted Cell Populations Cleared Heart Three-dimensional Imaging Endogenous Heart Regeneration Regenerative Response 3D Imaging Cellular Dynamics Cardiac Regenerative Response Clearing Techniques Staining Techniques Imaging Techniques LAD-induced Coronary Artery Occlusion Surgical Area Sterilization Transverse Skin Incision Intercostal Muscles Superficial Puncture Internal Organs Blunt Dissection Chest Cavity Extraction
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