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Perfiles de Complexome de Alta Resolución por Cryoslicing BN-MS Analysis

JoVE Journal
Biochemistry

This content is Free Access.

JoVE Journal Biochemistry

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

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Perfiles de Complexome de Alta Resolución por Cryoslicing BN-MS Analysis

6,967 Views

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09:33 min

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October 15, 2019

DOI:

10.3791/60096-v

DOI:

10.3791/60096-v

•

09:33 min

October 15, 2019

•

2 Views

Catrin Swantje Müller¹, Wolfgang Bildl¹, Norbert Klugbauer², Alexander Haupt¹, Bernd Fakler^1,3, Uwe Schulte^1,3,4

¹Institute of Physiology, Faculty of Medicine,University of Freiburg, Freiburg, Germany, ²Institute of Experimental and Clinical Pharmacology and Toxicology, Faculty of Medicine,University of Freiburg, Freiburg, Germany, ³Signalling Research Centres BIOSS and CIBSS, Freiburg, Germany, ⁴Logopharm GmbH, Germany

Summary

Se presenta un protocolo BN-MS crioscrioso versátil que utiliza un microtome para perfiles de complexomo de alta resolución.

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