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High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
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Bioquímica
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JoVE Journal Bioquímica
High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

6,967 Views

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09:33 min

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October 15, 2019

DOI:

10.3791/60096-v

DOI:
10.3791/60096-v

•

09:33 min
October 15, 2019

•

2 Views
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1Institute of Physiology, Faculty of Medicine,University of Freiburg, Freiburg, Germany, 2Institute of Experimental and Clinical Pharmacology and Toxicology, Faculty of Medicine,University of Freiburg, Freiburg, Germany, 3Signalling Research Centres BIOSS and CIBSS, Freiburg, Germany, 4Logopharm GmbH, Germany

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A versatile cryoslicing BN-MS protocol using a microtome is presented for high-resolution complexome profiling.

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CsBN-MSProtein ComplexesMembrane ProteinsSignal TransductionNative Protein ComplexSubunit CompositionGradient GelPolymerizationLoading SlotsRunning BuffersProtein Solubilization

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Müller, C. S., Bildl, W., Klugbauer, N., Haupt, A., Fakler, B., Schulte, U. High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis. J. Vis. Exp. (152), e60096, doi:10.3791/60096 (2019).

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