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クライオスライシングBN-MS解析による高解像度複合プロファイリング
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
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クライオスライシングBN-MS解析による高解像度複合プロファイリング

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6,885 Views

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09:33 min

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October 15, 2019

DOI:

10.3791/60096-v

DOI:
10.3791/60096-v

•

09:33 min
October 15, 2019

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1Institute of Physiology, Faculty of Medicine,University of Freiburg, Freiburg, Germany, 2Institute of Experimental and Clinical Pharmacology and Toxicology, Faculty of Medicine,University of Freiburg, Freiburg, Germany, 3Signalling Research Centres BIOSS and CIBSS, Freiburg, Germany, 4Logopharm GmbH, Germany

Summary

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マイクロトームを用いて汎用性の高い解起性BN-MSプロトコルを高解像度の複合プロファイリング用に提示する。

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CsBN-MSProtein ComplexesMembrane ProteinsSignal TransductionNative Protein ComplexSubunit CompositionGradient GelPolymerizationLoading SlotsRunning BuffersProtein Solubilization

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Müller, C. S., Bildl, W., Klugbauer, N., Haupt, A., Fakler, B., Schulte, U. High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis. J. Vis. Exp. (152), e60096, doi:10.3791/60096 (2019).

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