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Perfilamento Complexome de alta resolução por Cryoslicing BN-MS Analysis

JoVE Journal
Biochemistry

This content is Free Access.

JoVE Journal Biochemistry

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

Perfilamento Complexome de alta resolução por Cryoslicing BN-MS Analysis

6,973 Views

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09:33 min

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October 15, 2019

DOI:

10.3791/60096-v

DOI:

10.3791/60096-v

•

09:33 min

October 15, 2019

•

2 Views

Catrin Swantje Müller¹, Wolfgang Bildl¹, Norbert Klugbauer², Alexander Haupt¹, Bernd Fakler^1,3, Uwe Schulte^1,3,4

¹Institute of Physiology, Faculty of Medicine,University of Freiburg, Freiburg, Germany, ²Institute of Experimental and Clinical Pharmacology and Toxicology, Faculty of Medicine,University of Freiburg, Freiburg, Germany, ³Signalling Research Centres BIOSS and CIBSS, Freiburg, Germany, ⁴Logopharm GmbH, Germany

Summary

Um protocolo versátil de cryoslicing BN-MS usando um micrótomo é apresentado para o perfilamento complexome de alta resolução.

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