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High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
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生化学
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JoVE Journal 生化学
High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

DOI:
10.3791/60096-v

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09:33 min

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October 15, 2019

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, , , , ,
1Institute of Physiology, Faculty of Medicine,University of Freiburg, Freiburg, Germany, 2Institute of Experimental and Clinical Pharmacology and Toxicology, Faculty of Medicine,University of Freiburg, Freiburg, Germany, 3Signalling Research Centres BIOSS and CIBSS, Freiburg, Germany, 4Logopharm GmbH, Germany

章

  • 00:04標題
  • 01:16Preparative BN-PAGE
  • 03:44Gel Sampling and Digestion
  • 06:32Results: High-Resolution Complexome Profiling
  • 08:29Conclusion

概要

自動翻訳

自動翻訳

A versatile cryoslicing BN-MS protocol using a microtome is presented for high-resolution complexome profiling.

タグ

CsBN-MSProtein ComplexesMembrane ProteinsSignal TransductionNative Protein ComplexSubunit CompositionGradient GelPolymerizationLoading SlotsRunning BuffersProtein Solubilization

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