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葡萄糖-6-磷酸异构酶（G6PI）诱导RA小鼠中iNKT细胞的采用免疫治疗

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Immunology and Infection

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JoVE Journal Immunology and Infection

Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase (G6PI)-Induced RA Mice

Please note that all translations are automatically generated. Click here for the English version.

葡萄糖-6-磷酸异构酶（G6PI）诱导RA小鼠中iNKT细胞的采用免疫治疗

DOI:

10.3791/60048-v

•

08:43 min

•

January 31, 2020

•

Ming Meng*^1,2, Shengde Chen*^1,2, Xiang Gao*^1,2, Huifang Liu*^1,2, Yuanyuan Wang*^1,2, Jingnan Zhang*^1,2, Haiyang Dou*^1,2, Wenjuan Li*^1,2, Dongzhi Chen*^1,2

¹Medical School of Hebei University, ²Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-Autoimmune Diseases in Hebei Province

Chapters

00:04Introduction
01:15Constructing the Disease Model
02:32Obtaining iNKT (Invariant Natural Killer T) Cells with Adoptive Cellular Therapy
06:20Results: Changes in the Mice
07:25Conclusion

Summary

Automatic Translation

该协议使用G6PI混合肽构建类风湿关节炎模型，更接近^CD4+T细胞和细胞因子中的人类类风湿性关节炎模型。高纯度不变天然杀伤性T细胞（主要是iNKT2），具有特定的表型和功能，通过体内诱导和体外纯化获得，用于采用免疫治疗。

Tags

Adoptive Immunotherapy INKT Cells Glucose-6-phosphate Isomerase (G6PI)Rheumatoid Arthritis (RA) Model RA Pathogenesis T Cells B Cells Innate Immunity In Vivo Emulsification Pertussis Toxin Spleen Cell Isolation Lymphocyte Separation INKT Cell Purification

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