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Preparation of Tumor Antigen-loaded Mature Dendritic Cells for Immunotherapy
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Chapters
- 00:05Title
- 01:09Isolation and Cryopreservation of PBMCs
- 03:09Day 0: Differentiation of Dendritic Cells from Monocytes
- 04:33Day 5: Harvesting of Immature Dendritic Cells
- 05:21Day 6: Maturation and Antigen Loading of Dendritic Cells
- 05:58Day 7: Cryopreservation of Dendritic Cells
- 07:13Results: Poly-ICLC Treatment Induces PBMC Differentiation into Functionally Mature DCs
- 08:14Conclusion
The most commonly used method for generating large numbers of autologous dendritic cells (DCs) for use in tumor immunotherapy is described. The method uses IL-4 and GM-CSF to differentiate DCs from monocytes. The immature DCs are stimulated to mature and then loaded with antigens before they are injected back into the patient.
