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August 18, 2019
DOI:
1Laboratory of Molecular Biology, Department of Plant Sciences,Wageningen University & Research, 2Center of Technology for Agricultural Production,Agency for the Assessment and Application of Technology (BPPT), 3Department of Ecological Science, Faculty of Earth and Life Sciences,Vrije Universiteit Amsterdam, 4Beijing Advanced Innovation Center for Tree Breeding by Molecular Design,Beijing University of Agriculture
Summary
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Parasponia andersonii, Kenevir ailesine (Cannabaceae) ait olan ve rizobium ile birlikte azot sabitleme kökü nodülleri oluşturabilen hızlı büyüyen tropikal bir ağaçtır. Burada, Agrobacterium tumefaciens-aracılı kararlı dönüşüm ve CRISPR/Cas9 tabanlı genom düzenlemesine dayalı P. andersonii'de ters genetik analizler için ayrıntılı bir protokol açıklıyoruz.
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Wardhani, T. A., Roswanjaya, Y. P., Dupin, S., Li, H., Linders, S., Hartog, M., Geurts, R., van Zeijl, A. Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii. J. Vis. Exp. (150), e59971, doi:10.3791/59971 (2019).
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