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Transforming, Genome Editing and Phenoty the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii (en plus de la transformation, de l'édition du génome et du phénotypage de l'arbre tropical de Cannabaceae parasponia andersonii)
JoVE Journal
Genetics
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JoVE Journal Genetics
Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii
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Transforming, Genome Editing and Phenoty the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii (en plus de la transformation, de l'édition du génome et du phénotypage de l'arbre tropical de Cannabaceae parasponia andersonii)

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12,378 Views

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12:22 min

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August 18, 2019

DOI:

10.3791/59971-v

DOI:
10.3791/59971-v

•

12:22 min
August 18, 2019

•

12 Views
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1Laboratory of Molecular Biology, Department of Plant Sciences,Wageningen University & Research, 2Center of Technology for Agricultural Production,Agency for the Assessment and Application of Technology (BPPT), 3Department of Ecological Science, Faculty of Earth and Life Sciences,Vrije Universiteit Amsterdam, 4Beijing Advanced Innovation Center for Tree Breeding by Molecular Design,Beijing University of Agriculture

Summary

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Parasponia andersonii est un arbre tropical à croissance rapide qui appartient à la famille du cannabis (Cannabaceae) et peut former des nodules de racine fixant l'azote en association avec le rhizobium. Ici, nous décrivons un protocole détaillé pour les analyses génétiques inversées dans P. andersonii basé sur la transformation stable d'Agrobacterium-négociée et l'édition de génome CRISPR/Cas9-basée.

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Parasponia AndersoniiNitrogen-fixingTropical TreeCannabaceaeGenome EditingPhenotypingTransgenic Mutant LinesRhizobial SymbiosisIn Vitro PropagationSeed GerminationPlant Transformation

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Wardhani, T. A., Roswanjaya, Y. P., Dupin, S., Li, H., Linders, S., Hartog, M., Geurts, R., van Zeijl, A. Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii. J. Vis. Exp. (150), e59971, doi:10.3791/59971 (2019).

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