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Genetics

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JoVE Journal Genetics
Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii
 

Transforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii

Article DOI: 10.3791/59971-v • 12:22 min • August 18th, 2019
August 18th, 2019 •
1,2, 1,2, 1,3, 1,4, 1, 1, 1, 1
1Laboratory of Molecular Biology, Department of Plant Sciences, Wageningen University & Research, 2Center of Technology for Agricultural Production, Agency for the Assessment and Application of Technology (BPPT), 3Department of Ecological Science, Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, 4Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture

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요약
Automatic Translation

Parasponia andersonii is a fast-growing tropical tree that belongs to the Cannabis family (Cannabaceae) and can form nitrogen-fixing root nodules in association with the rhizobium. Here, we describe a detailed protocol for reverse genetic analyses in P. andersonii based on Agrobacterium tumefaciens-mediated stable transformation and CRISPR/Cas9-based genome editing.

Tags

Transforming Genome Editing Phenotyping Nitrogen-fixing Tropical Tree Parasponia Andersonii Cannabis Family Rhizobium Symbiosis Comparative Studies Genetic Networks Stable Transgenic Mutant Lines In Vitro Propagation Tissue Culture Molecular Cloning Research Model Germinating Seeds Disinfect Seeds Hypochlorite Bleach Sterilized Water PCR Tubes
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